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What is the expected size of the protein

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PERNICIOUS ANAEMIA PART - Western Blotting and calculation refresher

Question 1: What is the expected size of the protein being detected by the sera from pernicious anaemia patients?

Question 2: Which submit of the proton pump does this correspond to?

Question 3: Describe another method that could be used to detect and quantitate the levels of anti¬proton pump antibodies in patient sera?

Question 4: The secondary antibody that was used in the Western and immuno-histochemistry was a sheep anti-human Ig-HRP conjugate.

Question 5: What is the antigen that this antibody is specific for? What animal was this antibody raised in? What is the enzyme that is conjugated to the antibody?

Attachment:- Western Blotting and calculation refresher.rar

Reference no: EM133206608

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