What is the expected size of the protein

Assignment Help Science
Reference no: EM131603771

This is a scientific report. Do the introduction, Aims of practical, methods and reference parts. Questions and discussion parts. Top of that they gave some articles about the disease please read those articles as well. do your best.

Practical report

- Read the article entitled "Pernicious anaemia" (1997) by Toh, B.H., van Driel, I.R. and Gleeson, P.A., New England Journal of Medicine, 337, p1441-1448

- Obtain an entire set of results for all serum samples for the Western blotting and immuno-peroxidase staining from the other students at your bench. Summarise the results in a table.

- Determine which of the patients can be diagnosed with pernicious anaemia.

Avoid the temptation to cut and paste anything from the manual, journal articles or web pages. It is really obvious and easily detected by anti-plagiarism software.

Your Prac reports should include the following:

- Title and author: A title should reflect the report content. Below the title include your name and practical partner's name and practical session

- A short paragraph that describes the Aim/Aims of the Prac

- An Introduction that gives a background to the experiments. What is pernicious anaemia? Provide some background to the experimental procedure and what you hope to achieve. You can make reference to published articles here. This should be half to one page in length.

- A Methods section that summarises the main steps you followed with reference to the detail in the Prac manual. Do not include materials lists. You can use sub- headings for different techniques. Write in paragraph form, not dot-points. It should not look anything like the manual. The manual is full of unnecessary details. Make sure your methods reflect modifications made to procedures. Don't copy straight from the manual. If you used 200µL instead of 100µL of serum your methods should state the correct figure.

- The Results section should systematically describe your results in detail, with reference to Figures and Tables that display your data. All data presented in Figures or Tables, including diagrams, should also be commented on in the text of the Results section. Provide Figure Legends to accompany your Figures and a heading for Tables. You can use subheadings in this section if you like.

- In the Discussion you should discuss the significance of your findings. Explain what your Results mean and how they contribute to understanding pernicious anaemia. Mention if you achieved your Aims and if there were any factors that adversely contributed to the experiment. If you had technical problems and you did not obtain good results explain what occurred, give a scientific explanation for the failed result. Suggest how the problem might be remedied. Could you improve the experiment? Provide references to support your argument.

- In your Prac write-up, include the answers to the following questions in whichever section you think appropriate eg Introduction, Materials and Methods, Discussion etc:

Questions

a. What is the expected size of the protein being detected by the sera from pernicious anaemia patients?

b. Which subunit of the proton pump does this correspond to?

c. Describe another method that could be used to detect and quantitate the levels of anti-proton pump antibodies in patient's sera?

d. The secondary antibody that was used in the Western and immuno-histochemistry was a sheep anti-human Ig-HRP conjugate. What is the antigen that this antibody is specific for? What animal was this antibody raised in? What is the enzyme that is conjugated to the antibody?

- Refer to published papers (primary literature) as well as secondary literature in your Prac report and use Endnote to reference your write up
- Obtain electronic images of your mouse stomach diagrams from your phone or the Library scanner
- Ensure all figures and tables are labelled properly. Can they be interpreted without reading the text? Use legends where necessary.
- Word limit 2000-2500

Verified Expert

The total word limit of the assignment is 2300 which includes results, discussion and conclusion part as well. This part the client said, he would do. Which means I am left with Aims/objective, introduction and Methodology part which requires not more than 1500-1700 words. Hence, please confirm this from the client.

Reference no: EM131603771

Questions Cloud

Worth of securities from the non-bank public : Suppose that the Federal Reserve purchases $10,000 worth of securities from the non-bank public.
Evaluate strength and weaknesses of talk show guests claim : Evaluate the strength and weaknesses of the talk show guest's claim. What constitutes a major depression diagnosis. Two possible treatment for major depression.
Describe the experimental results in all the three condition : Describe the experimental results in all the three conditions. In addition, identify the condition where you performed the best and the worst.
Define what social work values are embraced : How do theoretical concepts translate into or guide specific practice goals and practice skills
What is the expected size of the protein : What is the expected size of the protein being detected - Which subunit of the proton pump does this correspond to?
Uncertainty plays a very significant role in forecasting : Financial planning is very important in today’s economy but uncertainty plays a very significant role in forecasting.
Complete an evaluation of your kitchen safety : Conduct a safety review of your kitchen in your house. Spend some time in your kitchen reviewing the process of how food is handled throughout the kitchen area.
What is the value of bond that matures in nine years : What is the value of a bond that matures in 9 years, has an annual coupon payment of $100, and has a par value of $1000?
What is the price of the floating-rate preferred stock : What is the price of the floating-rate preferred stock most likely to be? What is the price of the straight preferred stock likely to be?

Reviews

inf1603771

9/25/2017 6:13:59 AM

I guess 1700 words enough because I'm going to do the results and discussion part sorry about the late reply I just saw the solution. It looks great how many words are there? 1900 words doesn't matter. I read the solution, it seems great. Thank you so much.

len1603771

8/18/2017 1:34:45 AM

Hello guys, this is a scientific report. could you please do the introduction, Aims of practical, methods and reference parts. i will do the results, questions and discussion parts. Please read the practical and marking rubric carefully. on top of that they gave some articles about the disease please read those articles as well. do your best. thanks.

len1603771

8/18/2017 1:34:27 AM

Reference List (any common style used in Medical Science is acceptable) 0-0.4 points Missing in-text citation and reference list 0.5 -0.9 points Either in-text citations or reference list is missing 1-1.9 points Some references but poor formatting 2-2.9 points References complete, with variable formatting 3 points References complete with consistent formatting Overall Score Inadequate 0 or more Unsatisfactory 8 or more Fair 16.5 or more Good 23 or more Excellent 30 or more

len1603771

8/18/2017 1:34:21 AM

Discussion 0 -1.4 points Missing or incorrect. Does not interpret results. 1.5-2.9 points Discussion includes partial or oversimplified interpretation of Results 3-3.9 points Discusses the significance of most results with acceptable 4-4.9 points Good interpretation and mostly clear/correct discussion of 5 points Correct interpretation and clear discussion of significance of Results to a detail and complexity significance of Results high scientific level. Questions 0-1.4 points Missing, incorrect or incomprehensible 1-1.9 point Less than half the questions answered correctly. Insufficient detail. 2-2.9 points Half the questions answered correctly with good scientific understanding 3-3.9 points Most or all of the questions answered correctly, concisely and to a high scientific standard 4 points All of the questions answered correctly, clearly, concisely and to a high scientific standard

len1603771

8/18/2017 1:34:12 AM

Results text 0-1.9 points Missing or incorrect information provided 2-2.9 points Incomplete description of results or results contain material that should be in discussion 3-3.9 points Basic description of results with reference to figures and tables in the text 4-4.9 points Good description of results with reference to all figures and tables in the text 5 points Clear and accurate description of results with reference to all figures and tables Figures (including diagrams) and Tables 0-1.4 points Missing, Poorly presented/ mostly incomplete 1.5-2.9 points Some figures and tables missing AND/OR legends missing or incomplete 3-3.9 points All Figures and Tables including Figure legends and titles. Presentation average. 4-4.9 points Clear and mostly consistent presentation of Figures and Tables and fairly comprehensive Figure legends and titles 5 points Clear, well presented, consistent style for Figures and Tables and comprehensive Figure legends and titles

len1603771

8/18/2017 1:34:05 AM

Aims of the Prac 0-0.24 points Missing or incorrect 0.25-0.4 points Inappropriate, incomplete or unclear 0.5-0.9points Stated but not very clear, or copied verbatim from the manual 1-1.4 point Stated and reasonably clear 1.5 points Stated and articulated very well Materials and Methods 0-0.4 points Missing or incorrect 0.5-0.9 points Incorrect format for the materials and methods section eg lists. 1-1.9 point Either insufficient or too much detail present. Poor presentation 2-2.9 points Generally sufficient and accurate detail present. Good presentation 3 points Excellent detail present. Accurate. Exemplary presentation.

len1603771

8/18/2017 1:34:00 AM

PERNICIOUS ANAEMIA EXAMPLE RUBRIC – (Please check on CloudDeakin for the final version of the rubric as it may have been modified since this manual was published). Criteria Missing Unsatisfactory Fair Good Excellent Title, Author and Introduction to the Prac 0-0.9 points Missing title, author and/or Introduction 1 -1.9point Missing either title, author or Introduction 2-2.9points Title and Introduction missing, but Introduction not complete 3-3.9 points Title included. Introduction included and fairly comprehensive, referencing fair 4 points Title included and Introduction very well articulated, referencing good

Write a Review

Science Questions & Answers

  Address influence of internal and environmental conditions

Address the influence of internal and environmental conditions on what is recalled from certain kinds of memory/representations

  Compare the restoring force for capillary waves

Compare the restoring force for capillary waves versus that of longer wind-driven ocean waves. How does bathymetry affect a shallow-water wave such as a tsunami

  Describe four methods of learning and listening

Describe four methods to overcome communication barriers and provide detailed examples.

  Evaluate and contrast source reduction-emission reduction

Many photographic processes use chromium salts. Washing the photographic plates results in excess hexavalent chromium being discharged into wash waters and eventually into our waterways. Considering the pollution prevention hierarchy, what options..

  What effect will this have on planet earth

The red shift seen in all distant galaxies, whose size is proportional to its distance from us, what effect will this have on planet Earth?

  Define personal image and define maintaining professionalism

Define personal image and discuss why maintaining professionalism while on the job and after hours are important. Give at least two examples of ways health care providers exhibit professionalism in practice.

  Great deal of trouble initiating and maintaining sleep

Fred has been having a great deal of trouble initiating and maintaining sleep

  Describe the role the patient history and physical exam

Select one of the four case studies provided. Reflect on the provided patient information including history and physical exams. Think about a differential diagnosis. Consider the role the patient history and physical exam played in your diagno..

  The central values and beliefs set forth by the theorist

What in the background of this theorist as a nursing scholar might have prompted her to develop the theory?

  Causes and mechanismnbspof stratospheric ozone destruction

answer all three questions belowa. causes and mechanismnbspof stratospheric ozone destruction. provide the caa

  What environmental problems do open dumps cause?

Describe a modern landfill.What are the differences as compared to early landfills?

  Carbohydrate as a monosaccharide or disaccharide

What did you learn that you did not know before? In what way will it influence the food choices you make now?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd