What is the equilibrium constant keq for the reaction

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Recently, protein A was discovered to bind ligand B, forming complex AB as depicted below.

A+B?AB

After mixing a solution of 1 μM [A] and 2 μM [B], you allow the mixture to incubate at room temperature (22 C) until the reaction reaches equilibrium. You then measure that [AB] is 990 nM. Also, no protein degradation, aggregation, etc. has occurred

  1. What is the equilibrium constant Keq for the above reaction?
  2. What is the change in Gibbs free energy associated with this reaction (R gas constant = 8.314 Joules/mol K)?

Reference no: EM132439555

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