What is the correct structure of the native protein

Assignment Help Biology

Reference no: EM13121602

A protein has a molecular mass of 200 kDa as determined by gel filtration (=native molecular weight). When separated by SDS-PAGE (without a reducing agent present) only one polypeptide appeared with a mass corresponding to 100 kDa. When determined by SDS-PAGE in combination with a reducing agent (DTT) exactly two polypeptides appeared of sizes 40 and 60 kDa. What is the correct structure of the native protein?

Select one:

a. The native protein is made of one 40 kDA and one 60 kDA polypeptides
b. The native protein is made of two 100 kDa polypeptides
c. The native protein is made of two 40 kDA and two 60 kDA polypeptides
d. The native protein is made of two 100 kDa polypeptides, one 40 kDA and one 60 kDA polypeptides
e. None of the answers is correct.

Reference no: EM13121602

Questions Cloud

What is the partial pressure of helium : A 295mLflask contains pure helium at a pressure of 742torr . A second flask with a volume of 465mL contains pure argon at a pressure of 717torr .

Find probability to not be able to make the payment : Consider a charity organizations that receives random donations. Assume the donations to arrive following a Poisson process with a rate of one donation per hour in average. What is the probability that it will not be able to make the payment?

Relationship between inflation and unemployment rates : Economic data has shown a relationship between Inflation and Unemployment rates. What is this relationship called? What are some determining factors of the relationship? How has government fiscal/monetary policy been utilized to attempt to manage ..

Maslow''s theory seems to fit for much of behavior : Maslow's theory seems to fit for much of behavior, but what about people who go on hunger strikes to meet higher order needs or who risk their own safety to protect their children?

What is the correct structure of the native protein : A protein has a molecular mass of 200 kDa as determined by gel filtration (=native molecular weight). When separated by SDS-PAGE (without a reducing agent present) only one polypeptide appeared with a mass corresponding to 100 kDa.

What is the ph of this solution : A buffer (pH 3.70) was prepared by mixing 1.00 mole of formic acid and 1.00 mole of sodium formate to form an aqueous solution with a total volume of 1.00 L.

Which one would show up as the band at the bottom of the gel : Four proteins, each containing a single polypeptide, are separated by SDS-PAGE: human cytochrome c (MW 13,000), human serum albumin (MW 67,000), bovine myoglobin (MW 16,890), and human apolipoprotein B (MW 513,000). Which one would show up as the ..

Find the probability that all six professors are extraverts : It is reported that 45% of all college professors are extroverted. Suppose you have classes with six different professors. What is the probability that all six are extroverts?

What increase in axonal permeability to sodium cause : What would the sudden increase in axonal permeability to sodium cause?

User Account

All Pages