What is the concentration of the rna in the cuvette

Assignment Help Biology

Reference no: EM13145178

You have purified total RNA from leaf cells of a maple tree. You dissolved all the RNA in 50 ul (microliters) of pure water to make an "RNA Sample." You need to determine the concentration (ug/ul) of this RNA sample, to do this you removed 2 ul from the sample and added these to 998 ul of water and put in a cuvette. You determined the absorbance of light at 260nm for this 1ml of RNA water solution in the cuvette and the A260 value was 0.058. A260 of 1.0 = 40 ug/ml for RNA.

i) what is the concentration (ug/ul) of the RNA in the cuvette?

ii) what is the concentration (ug/ul) of the RNA in the original RNA sample?

iii) How many ul of the RNA sample would you need to use in an experiment that required a total of 10ug RNA?

Reference no: EM13145178

Questions Cloud

Why is it important to track inventory : What activities should you perform to correctly account for your inventory at year-end? Why is it important to track inventory? What does this information tell you about your business?

Recognizd gain or loss : On April 18, 2010, Jane Junipner purchased 30 shares of Bryan Corp. stock for $210, and on September 29, 2010 she purchased an additional 90 shares for $900. On November 28, 2010, she sold 48 shares which could not be specifically identified, for ..

Find probability that mouse will live thirty two months : Find the probability that a given mouse will live. a. more than 32 months b. less than 28 months c. between 37 and 49 months.

What is the minimum amount of hydrogen gas in grams required : What is the minimum amount of hydrogen gas in grams required to completely hydrogenate 12.5 Kg of 2-butene?

What is the concentration of the rna in the cuvette : You have purified total RNA from leaf cells of a maple tree. You dissolved all the RNA in 50 ul (microliters) of pure water to make an "RNA Sample.

What is gain recognized on this reorganization : It only used $200,000 of the cash and the remaining cash and P stock are distributed to T’s shareholder, who has a basis of $15 million in her stock. What is gain recognized on this reorganization?"

Entry to record the exchange of assets : Lupa Inc. trades its used machine (cost $12,000 less $4,000 accumulated depreciation) for a new machine. In addition to exchanging the old machine (which had a fair market value of $9,000), Lupa also paid cash of $3,000.

Calculate the density of the solid : A 32.65-g sample of a solid is placed in a flask. Liquid toluene, in which the solid is insoluble, is added to the flask so that the total volume of solid and liquid together is 50.00 ml.

What is the advantage of the respective properties : Cotton and Potatoes. A cotton fiber consists almost exclusively of cellulose, whereas a potato tuber contains mainly starch. Cotton is a tough, fibrous, and virtually insoluble in water.

User Account

All Pages