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You have purified total RNA from leaf cells of a maple tree. You dissolved all the RNA in 50 ul (microliters) of pure water to make an "RNA Sample." You need to determine the concentration (ug/ul) of this RNA sample, to do this you removed 2 ul from the sample and added these to 998 ul of water and put in a cuvette. You determined the absorbance of light at 260nm for this 1ml of RNA water solution in the cuvette and the A260 value was 0.058. A260 of 1.0 = 40 ug/ml for RNA.
i) what is the concentration (ug/ul) of the RNA in the cuvette?
ii) what is the concentration (ug/ul) of the RNA in the original RNA sample?
iii) How many ul of the RNA sample would you need to use in an experiment that required a total of 10ug RNA?
List the three types of chemical bonds (from weakest to strongest), and describe how each type is formed.
What is a good Hypothesis for What's my problem? Is it ADD or is it ADT?
Upon entering graduate school, you heard rumors about a student, who was totally inspired by a "rusty nail experience".
Two highly inbred strains of mice are crossed, and the FI generation has a mean tail length of 5 cm and a standard deviation of 1.5 cm. The F2 generation has a mean tail length of five cm and a standard deviation of four cm.
Roges Staubauch has severe arthritis and has been taking prednisone for two months. He isn't feeling well, complains of repeated colds, and has become really puff in his appearance. What is the reason for this symptoms.
It has been shown that the repair of UV induced dimers in a gene is coupled to transcription. Suggest a model for this and any experiments you would use to test your model.
Many scientists were reluctant to accept the results of Avery, MacLeod, and McCarty's experiments showing that DNA (rather than protein) was the transforming principle. Why?
What affect might you expect the precipitation of hemoglobin S and the subsequent cell sickling to have on oxygen delivery in an individual with sickle cell disease?
Two of the embryonic sections differentiate resulting in 5 sections to the brain. The thalamus as a part of diencephalon develops from which initial embryonic brain section? (note there are three embryonic sections from which five sections diverge..
List two types of macromolecules that have been partly digested by the time acid chyme moves into the intestine. Where did this digestion take place and what enzymes were involved.
lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome.
Describe why topoisomerases are required in eucaryotic cells even though eucaryotic DNA molecules are linear, not circular like procaryotic DNA molecules.
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