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My professor was not very explicit on exactly what she wanted. We are supposed to show our calculations on how to make these solutions for lab.
1) 500 ml of 75% ethanol (v/v) from 100% stock (200 proof).2) 50 ml of 5mM Tris pH 7.4 (from 1M stock), 150mM Nacl, 0.05% (v/v) Triton X-100.3) A 10X buffer contains 200mM Tris, 1.5M Nacl, and 10% SDS:
a) How would you make 500 ml of 1X buffer?b) What is the concentration of each component of the 1X buffer?
In cells, the phosphorylation of glucose is the first step in glucose metabolism and is accomplished by addition of phosphate from ATP which generates ADP and Pi (G° = -7.3 kcal/mole). Therefore, in the cell there are two reactions coupled togethe..
Marie and her brother, Donnie are healthy adults. Marie and her healthy boyfriend, Dweezil, have a healthy baby girl. Marie is pregnant again. Marie and Donnie's mother had a baby by a second marriage to a healthy male.
Why is replication host range important when considering horizontal gene transfer mediated by plasmids.
Each cell of the human body contains 46 chromosomes. a. How many double-stranded DNA molecules does this statement represent? b. How many different types of DNA molecules does it represent?
A restriction endonuclease "cuts" two DNA molecules at the same location. What can you assume is identical about the molecules at that location?
Ebony is a seven year old child who didnt feel well and was complaining of a sore throat. By the next morning, Ebony had a bright red tongue and a rash across her chest.
What will the instructor tell the students are the most ordinary cause of an MI.H ow various different antibody molecules potentially could be generated.
Epithelial tissue covers all inner and outer: A. surfaces B. spaces C. ears D. nervous systems D. any of the above could be equally valid.
After the potato strips have been in their respective test tubes for 1 hour, describe the condition of the A strips (limp vs crisp) compared to the B strips.
What experiment could be performed to ascertain if an antimicrobial agent is bactericidal or bacteriostatic? Can a bacteriostatic agent become bactericidal in the long run or vice versa? Why?
What is meant by the 'cloning of a gene'? (Explain what it means from the perspective of the host bacteria)
Which of the following is not an advantage of multicellularity?
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