What is the concentration of each component

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Reference no: EM13161593

My professor was not very explicit on exactly what she wanted. We are supposed to show our calculations on how to make these solutions for lab.

1) 500 ml of 75% ethanol (v/v) from 100% stock (200 proof).
2) 50 ml of 5mM Tris pH 7.4 (from 1M stock), 150mM Nacl, 0.05% (v/v) Triton X-100.
3) A 10X buffer contains 200mM Tris, 1.5M Nacl, and 10% SDS:

a) How would you make 500 ml of 1X buffer?
b) What is the concentration of each component of the 1X buffer?

Reference no: EM13161593

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