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You have a cell suspension containing 4 * 10^5 cells/ml and want to seed these cells on microcarriers at a density of 1* 10^4 cells/cm^2. The average diameter of the microcarriers is 157.5um (the diameter range supplied by the vendor is 115-200um).What is the average surface area of the microcarriers?What number of microcarriers should you use to obtain an average surface area of 100cm^2?What volume of your cell suspension should be added to achieve the density given, using the surface area of 100cm^2?
What are the causes of gastroesophageal reflux disease (GERD)? What is the typical prognosis for GERD and how is it treated.
If a population with two alleles is at Hardy-Weinberg equilibrium and the frequency of one allele is 0.6, what is the heterozygote frequency.
How can altering ice nucleation protein production yield a bacterial strain useful to agriculture? Explain the logic and how this works.
A 19 year old college student cuts his lower lip while saving and seek medical advice. Physical examination confirms a deeps tissue wound that exhibits the cardinal signs of inflammation. In response to collagen and other basement membrane protein..
Would a false positive from the reaction between the inoculating loop and hydrogen peroxide be caused by poor specificity or poor sensitivity of the test system? Explain.
What is a chloride reversal potential. Is there a net movement of chloride into or out of the cell while you add GABA to the bath? If so, in which direction.
Think about a cross between a male who has vitamin D resistant rickets and a female who has fully functional PHEX protein and does not express vitamin D resistant rickets.
Suppose if ICF (K+) ion concentration is 165 mM, what ECF (K+) concentration is required for the condition of no net passive flux of K+ across the membrane at Vm = - 66 mV?
Assume you have found a new Xenopus gene that codes for a RNA binding protein. You are not sure what its function is but you are interested in finding out as much as you can.
Animals can be classified according to their mode of thermoregulation. Sort the animals below, indicating their likely thermoregulatory strategy. Note that poikilotherms are sometimes referred to as heterotherms.
in 1990, the first human gene therapy experiment on a patient with adenosine deaminase deficiency was done. Patients who are homozygous for a defective gene for this enzyme have defective immune systems and risk death from diseases as simple as a ..
Sinorhizobium meliloti can be protected from osmotic stress by the presence of certain disaccharides. Dan Orr found that sucrose does a fine job as an osmoprotectant for our strain of S. meliloti,
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