Reference no: EM132604499

1) What is sickle cell trait? What genotype is associated with sickle cell disease, and what genotype is associated with sickle cell trait? Remember that a genotype includes two alleles.

2) How can the sickle cell genotype of individuals be determined?

3) What does the abbreviation PCR stand for?

4) What is the general purpose for using PCR?

5) List 4 applications of PCR:

6) What are the 4 main ingredients of a PCR reaction? Define each.

7) Where does the DNA polymerase used for PCR come from? What is unique about this enzyme and why is it commonly used in PCR?

8) Which ingredient of a PCR reaction determines the specificity of the PCR reaction, in other words, which ingredient determines what DNA fragment will be amplified?

9) What are the 3 main steps of PCR? At what temperature is each step performed?