What is required to use blunt ended fragments

Reference no: EM13110184

Some restriction enzymes produce fragments that have single stranded ‘sticky ends', while other enzymes do not leave single stranded ends.

a. Give at least one situation in which you would want a SS sticky end on restriction fragments.

b. What is a situation in which you would not want sticky ends.

c. What is required to use blunt ended fragments (those without sticky ends) in a gene cloning protocol. What do you do differently compared to a stickyend cloning.

Reference no: EM13110184

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