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1. In terms of protein purification, explain what is meant by the terms yield and purity.
2. In terms of protein purification, explain what is meant by recovery? Why might it not be 100%?
3. Often in protein purification an active enzyme is the desired result. In this case what do the terms total units and specific activity refer to?
4. Do you expect the GFP purified by IMAC to be 100% pure? Why might there be non-specific binding to the nickel agarose beads?
5. The Bio-Rad DC assay is based on the Lowry protein assay. What is the principle of the Lowry protein assay?
6. What is the excitation and emission maxima of EGFP? How could we use these properties and an EGFP standard to determine amount of GFP we have in our fractions from the IMAC purification of GFP?
Which of the following is the direct product of DNA replication.
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