What is meant by a control plate

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Reference no: EM133472114

Assignment:

1. On which of the plates would you expect to find bacteria most like the original non-transformed E. coli colonies you initially observed? Explain your predictions.

2. If there are any genetically transformed bacterial cells, on which plate(s) would they most likely be located? Explain your predictions

3. Which plates should be compared to determine if any genetic transformation has occurred? Why?

4. What is meant by a control plate? What purpose does a control serve?

5. If a fluorescent green color is observed in the E. coli colonies then a new question might well be raised, "What are the two possible sources of fluorescence within the colonies when exposed to UV light?" Explain:

6. Recall what you observed when you shined the UV light onto a sample of original pGLO plasmid DNA and describe your observations.

7. Which of the two possible sources of the fluorescence can now be eliminated?

8. What does this observation indicate about the source of the fluorescence?

9. Describe the evidence that indicates whether your attempt at performing a genetic transformation was successful or not successful. Look again at your four plates. Do vou observe some E. coli growing on the LB plate that does not contain ampicillin or arabinose?

10. From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? Explain your answer.

11. How would you change the bacteria's environment the plate they are growing on--to best tell if they are ampicillin resistant?

12. Very often an organism's traits are caused by a combination of its genes and its environment.

13. Think about the green color you saw in the genetically transformed bacteria:

?. What two factors must be present in the bacteria's environment for you to see the green color? (Hint: one factor is in the plate and the other factor is in how you look at the bacteria).

b. What do you think each of the two environmental factors you listed above are doing to cause the genetically transformed bacteria to turn green?

c. What advantage would there be for an organism to be able to turn on or off particular genes in response to certain conditions?

Reference no: EM133472114

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