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1, Explain what a restriction enzyme is ans what it does?2, What are a restriction enzyme recognition site? in nature, what is the purpose of restriction enzymes?3, Discuss two ways that restriction enzymes are used in molecular genetics4, What is gel electrophoresis?5, What are DNA fingerprints used for?
Determine the number of significant digits in each number and write out the specific significant digits.
Early in development, most human cells turn off expression of essential component of telomerase, enzyme responsible for addition of telomere repeat sequences to ends of chromosomes.
As the human population continues to grow, what should we expect to happen to other species?
In the figure below, suppose the left-hand individual is selfed. What pattern of radioactive bands would you see in a Southern analysis of the progeny?
Assume that a dihybrid F2 ratio, resulting from epistasis, was 9:3:4. If a double heterozygote was crossed with the fully recessive type, what phenotypic ratio is expected among the offspring?
What is the habitat of most of the species of Escherichia? How do some strains of Escherichia become pathogenic? What are 2 diseases that are caused by different strains of Escherichia?
A 10ml suspension of cr51 labelled red blood cells containing 3 x 108 cpm total radioactivity was injected into a subject, after 10minutes a small blood sample was taken ,found to contain 5x104 cpm, calculate the total blood volume the individual?
Specify whether the taste of fresh water surprised you or not? Explain if your perception of coarseness change or not?
In a separate piece of paper, make a simple family tree for a couple showing children, and lineages from two children showing grand children, great grandchildren, great great grandchildren, and great great great grandchildren.
What is an electrogenic pump and what are some examples of electrogenic pumps in the biological systems?
Describe the regulation of eukaryotic gene expression. Include what regions of DNA and what proteins are important and why? Include a discussion of post-transcriptional regulation.
Compare the elution lane to the positive control lane. How do you know the recombinant protein present in the elution sample? How pure is the elution sample compared to the control?
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