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1) Describe how cDNA is produced, clearly indicating the relationship between a gene, an mRNA, and a cDNA?
2) What is an EST? How is an EST different from a genomic sequence? How is an EST different from a cDNA sequence? What is the EST database? What is a UniGene sequence (the Unigene database if you prefer)?
3)Describe a microarray and how it works? Indicate what is fixed to a support and what is used to probe the microarray. How does a microarray differ from a Northern blot?
Examination of a large sample of individuals from the population reveals there is six times as many heterozygote as homozygote recessive individuals in this populaion. What is the frequency of the recessive allele?
Several main types of animals are now extinct. A treasure trove of fossils from these unusual animals was found in the Canadian Rockies.
Create a simple diagram of the N cycle in a watershed using boxes and arrows; no need for numbers and do not forget to include important pools and fluxes.
Determine the other components of the master mix besides nucleotides and what are their functions?
What would be TRC for a male with the genotype AABbCcDD? For a female. In contrast, each dominant allele in an individual's genotype contributes 12 ridges to the TRC in males and 10 ridges to the TRC in females.
Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky" and why is this especially useful for inserting DNA fragments into plasmid DNA?
Rifamycin and actinomycin D are two antibiotics derived from bacterium Streptomyces. Rifamycin binds to b subunit of E. coli RNA polymerase and interferes with formation of the first phosphodiester bond in the RNA chain.
Single stranded binding protein, topoisomerase and telomerase all solve potential problems in DNA replicatio.
In insulin mRNA, the protein coding sequence has 318 nucleotides. How many amino acids would be translated from this sequence? How many amino acids would be in the C peptide?
How could these three cell types be clearly and simply distinguished from each other in an experimental laboratory and how and where do these different cell types differentiate to their mature functional characteristics?
Gene frequencies for the ABO alleles vary geographically. For example the I^A allele makes up nearly 50 percent of the genes for blood type among Australian Aborigines, about 25 percent in Central Asian populations,
Assume you are studying a microorganism from a hot spring that uses organic carbon as an energy and carbon source.
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