What is a chloride reversal potential

Assignment Help Biology

Reference no: EM136341

Q. The chloride concentration in the ACSF used in a lab is 131.5 mM, as the concentration in the internal solution used to fill patch electrodes is 4.4 mM. Suppose that you are making a whole cell current clamp recording from a cell using these solutions. You are recording at room temperature (20oC) and have determined that your cell has a resting potential of -70 mV.

A. What is a chloride reversal potential? Show the work.

B. If you rapidly add GABA to the bath, will GABA depolarize, hyperpolarize, or not alter the membrane potential? Describe.

C. Is there a net movement of chloride into or out of the cell while you add GABA to the bath? If so, in which direction?

D. presume you switch your internal solution to one containing 25 mM chloride and obtain a whole cell recording from a new cell that is also resting at -70 mV. What effect will the addition of GABA to the bath have on the membrane potential of this cell? On net direction of chloride movement into or out of cell?

Reference no: EM136341

Questions Cloud

Define a dimple in chin is a dominant characteristic : A man with a chin dimple and a non dimpled woman produce eight children, all having the chin dimple. Can you be certain of the man's genotype? Why or why not? What genotype is more likely, and why gives reason.

Discuss any differences from greek and roman health care : Male frogs give calls that attract female frogs to approach and mate. Researchers examined mating calls of closely related tree frogs in South America. What outcomes be able to occur where the ranges of two species overlap.

What is the initial stimulus : Is this an instance of a positive or negative feedback mechanisms? What is the initial stimulus? What is the result.

A phylogenetic tree represents a hypothesis : If Penicillium typically secretes penicillin without disturbing the lichen relationship in which it is engaged, then what should have been true about its partner.

What is a chloride reversal potential : What is a chloride reversal potential. Is there a net movement of chloride into or out of the cell while you add GABA to the bath? If so, in which direction.

Why is the man a major candidate for a heart attack : What is the possibility that the son would have both the Xg antigen and ichthyosis. Why is the man a major candidate for a heart attack.

What will be a tentative diagnosis and suggested treatment : The both endocrine and the nerve system are major regulating system of the body; however, the nervous system has been compared to an airmail system and the endoncrine system to the pony express. What is the resemblance.

Which of these represents the treatment option : Both RT and PR represent potential targets for antiretroviral drugs. Drugs called nucleoside analogs (NA) act against RT, whereas drugs known as protease inhibitors (PI) act against PR.

After system reaches equilibrium, what changes are observed : The mitochondria in them have a protein called thermogenin, which allows the flow of hydrogen ions through an inner mitochondrial membrane (bypassing the ATP synthase). Based on this and your understanding of cellular respiration, propose a mechanism..

User Account

All Pages