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A Western Blot (immunoblot) can be used as both a direct and as an indirect test. Explain how, comparing it first to a direct ELISA, and then to an indirect ELISA. Where do the antibodies and antigens come from in each case? What information does each test provide?
Consider the following peptide: Asp-Lys-Tyr-Glu-His-Cys-Glu-Lys-Ala-Arg-Cys.
They repeated the study with a larger number of samples but got the same results. What may be among their conclusions.
You have T cells that can respond to antigen A. Your spouse does not have T cells that respond to antigen A, so you offer to transfuse some of your T cells to your spouse. Why wouldn't it work? Be very specific.
The researchers concluded that at some time in the past, a slug ate algal cells and their DNA was somehow incorporated into the slug's DNA. This process is called horizontal gene transfer and may have happened numerous time in many different speci..
The rule of independent assortment states that alleles of one gene can segregate independently of alleles of other genes. In other words, the allele you pass on for one trait (for example, eye color) does not have any effect on the allele you pass..
You cross an individual that is PpCc with an individual that is homozygous recessive at both loci. Assuming independent assortment, what are the expected genotypic proportions?
Suppose that 2-pigments, red and blue, mix to give the normal wild-type purple color of petunia petals. Separate biochemical pathways synthesize the two pigments.
If C-1 of glucose is radioactively labeled & then enters glycolysis, which carbon of G-3-P will be labeled? Determine what fraction of the molecules will carry radioactive label?
XPA is a recessive DNA repair disorder that causes skin cancer with even the smallest exposure to sunlight. Heterozygotes are carriers, but are not affected.
The transfer of electrons from one molecule of NADH to H2O via the electron transfer chain results in the net movements of 8 protons from the mitochondrial matrix into the intermembrane mitochondrial space.
Eye color is expressed thrugh two genes A and B. Dark brown eye color, A, is dominant over lighter brown a. Eye pigment, B, is dominant over lack of eye pigment, b.
You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5mM in a final volume of 250mL?
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