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Explain (include a diagram if you wish) how the activity of protein kinase A (PKA) is regulated by binding of cAMP. Include in your explanation:
•How the term "consensus sequence" (-RRX(S/T)Y-) relates to the activity of PKA where X is a small amino acid residue (e.g., G), and Y is a large hydrophobic residue (e.g., I), making it clear that you know how the term relates to the function and activation of PKA; •the term "pseudosubstrate sequence";
•where in the structure of PKA cAMP binds;
•what happens to the structure of PKA when cAMP binds and how that relates to PKA activity.
There has not been rain for many months. A biologist is driving down the highway and notices that a big number of corn fields have corn that is wilted and short.
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Suppose that the each replication fork moves at a rate of 500 base pairs per second, how long would it take to replicate the E. coli chromosome from a single origin of replication?
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What would DNA footprinting reveal about the DNA binding sites of a normal Lac repressor protein? A normal RNA polymerase.
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Create a protocol to isolate and identify two different species of Enterobacteriaceae, Salmonella and E. coli.
A mutation occurs in Gene A that prevents RNA transcription from terminating where it is supposed to. Explain what affect, if any, you believe this mutation will have on.
According to the beer Lambert law, there should be a linear relationship between enzyme concentration and reaction rate. what possible explanations may be made for deviation from the linear relationship at higher enzyme concentration.
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