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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentraion of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
A 22 year old woman suffered a fracture of the femur in an automobile accident. Two weeks later, the bone defect contains vaxularized, fibrous tissue and newly formed, irregular bone spicules. Which of the following terms best describes this repai..
In andelusian chickens, the color of the feathers is cocominant. The extremes are black and white with mixed alleles creating blue. If the chickens are dominant for a long spur and recessive for a short spur, what are the genotypes and phenotypes ..
What structures in cells indicate an evolutionary relationship between bacteria, plants and animals?
The author of your text describes the gene for sickle cell anemia as being recessive. I prefer to think of it as codominant. What is there about this gene and the way it is expressed that it would best be described as codominant?
What happens to a virus that uses the rolling circle model of replication but has no means to cut monomers at discrete sites and cuts only unit lengths?
Many organisms possessing many different lipases produce positive results on Tributyrin Agar. Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity?
Consider the HIV enzyme called protease. The amino acid residues at the active site are highly hydrophobic. In designing a drug that would bind to the active site and jam it, researchers should use which sort of molecule.
What is the major component of cell walls in bacteria? How does this differ from plants? What are two sugar components and 3 amino acid components.
Diseases caused by eukaryotic parasites are much harder to treat than bacterial infections. What is the reason?
The antibiotic Gramicidin A can transport Na+ ions into a certain cell at the rate of 5.0 x 10^7 Na+ ions channel^-1 s^-1. Calculate the time in seconds to transport enough Na+ ions to increase its concentration.
ATP is a source of free energy that drives unfavorable reactions. a) Which of the following processes are coupled to the dephosphorylation of ATP?
how many molecules of nadh and fadh2 are produced from the breakdown of glucose and laureate?
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