Reference no: EM132934065

1. As Transcription starts, what Enzyme is evolved to separate the two strands that make up DNA?   

2. Enter the final nucleotide sequence for the mRNA molecule that is made using the DNA template on your screen. What is this mRNA sequence?  

3. Next, you will need to go through the Translation Process. What is the start codon?

4. Next, you will drag the correct amino acids to match up with the mRNA codons that appear on your screen. What is the final amino acid sequence you built? (Choose one of the genes on the screen)

5. Critical thinking: By bringing amino acids to the mRNA you are playing the role of which molecule involved in translation(highlight the correct answer below):

i. DNA

ii. Ribosomes

iii. tRNA

iv. Helicase

Exercise 2: Extracting DNA from cells

DNA extraction is a procedure that is used to remove and isolate DNA from cells. In the field of forensics, DNA must be isolated from the cell so that investigators can then use DNA analysis techniques to identify victims and criminals involved in crimes. In the field of genetic engineering, DNA must be isolated from cells so that it can be manipulated. Isolated DNA can also be used by genetic counselors for genetic testing of their patients.

A. DNA Extraction

Go to the "DNA extraction" activity at: https://learn.genetics.utah.edu/content/labs/extraction/

• Read the introductory paragraph, then click "start lab" in the virtual lab simulation. You can press "next" to advance or "back" to go back and re-watch animations. Follow the instructions in the lab while answering the following questions.

6. Why do you need human DNA?

7. On what part of the body are you collecting cells from the human subject?

8. What are the four steps of DNA extraction?

9. What tool did you use to collect your initial sample of DNA from the human test subject?

10. A micropipettor is used to measure and transfer very small volumes of liquid, anywhere from .01 microliters to 5 milliliters. You used it to add lysis solution to your initial DNA sample. Fill in the blank: Lysis is a Greek word that means "to ___________."

11. What is the purpose of the detergent part of the lysis solution?

12. What are some examples of detergents you may use in your home?

13. Fill in the blanks: The DNA is still wrapped very tightly around proteins called _________, and the ____________ enzyme cuts them apart to free the DNA.

14. Why did you add salt solution to the tube?