Reference no: EM132933554

Objective: Microscopy.docx https://www.youtube.com/watch?v=SUo2fHZaZCU

These resources are aimed at helping you become familiar with a microscope so that if you use one in the future, you will be well prepared. 

Please watch the video and read through the document describing microscopy. Once you have finished, please answer the questions listed below.

This video gives you an idea of what current biologists are doing with microscopes. It's super cool and is showing us what cells actually look like. 

https://www.youtube.com/watch?v=9euW5iCjKDo

Questions:

1. What objective lens is best when you are first starting out on a slide? Why?
2. What does 'Par focal' mean? Why is it useful?
3. The equation for the Numerical Aperture is NA=nsinθ. What does 'n' stand for? What does 'θ' stand for? If we want to get a better resolution, should we increase or decrease these values? Feel free to refer back to the lecture slides.
4. Using your textbook or other sources, please describe the following types of microscopy. In your description, please briefly explain how they work and why you would use them to look at a sample.

Electron microscopy

Confocal microscopy

Darkfield microscopy

Fluorescent microscopy