What does flaming the loop do

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Reference no: EM132763982

BIO410 Scientific Skills - Solent University

QUESTION ONE

To which bin would you discard your used culture plates? Tick the correct line.

	Sharps
	Clinical waste (yellow/ orange bag)
	Autoclave waste (transparent bag)
	General waste (black bag)

QUESTION TWO

To which bin would you discard your used microscope slides? Tick the correct line.

	Sharps
	Clinical waste (yellow/ orange bag)
	Autoclave waste (transparent bag)
	General waste (black bag)

QUESTION THREE

Some of the reagents we use in the lab are carcinogens. Please circle the hazard symbol that would be required on the reagent bottle to indicate this danger.

QUESTION FOUR

You have used Bradford's reagent in your practical classes this year. Bradford's reagent is irritant, corrosive and toxic. Please circle the hazard symbols that would be required on the reagent bottle to indicate these dangers.

QUESTION FIVE

Looking at the image of the microscope, list the names and/or functions of the labelled parts using the table on the next page.

	Microscope parts
1
2
3
4
5
6
7
8
9
10

QUESTION SIX

What major source of contamination is reduced by using a Bunsen burner?

QUESTION SEVEN

When you pick a bacterial colony to streak out onto a new plate to give single colonies, what does flaming the loop do?

QUESTION EIGHT

You are required to prepare a series of standards of bovine serum albumin (BSA) for a Bradford assay. You are given a BSA stock (concentration of 1.5 mg/mL) and phosphate buffered saline (PBS) to dilute the samples. The final concentrations of each of the five samples are listed in the table below, which should each have a final volume of 30 μl.

Calculate the volumes of BSA and PBS required for each sample.

Sample number	Final concentration BSA (mg/ml)	Volume BSA stock (μl)	Volume PBS (μl)
1	0	0	30
2	0.25	5	25
3	0.5	10	20
4	1.0	20	10
5	1.25	25	5

QUESTION NINE

If you were to analyse these samples in a spectrophotometer, please name the blank control solution you would use.

QUESTION TEN

At what point would you use the blank control?

QUESTION ELEVEN

What absorbance measurement should you get for the blank control?

QUESTION TWELVE

If you were to draw a graph of the results, what would you plot on the:

a) y-axis

b) x-axis

QUESTION THIRTEEN

What are the units for the x-axis?

QUESTION FOURTEEN

In micrometry, you see this image using a stage micrometer on the x4 objective lens.

The upper line is the eye piece graticule, the lower line is the stage micrometer. You know the micrometer is 1000μm long and has 100 divisions in total. Please calculate the length of each graticule division. Please show your working and units as this will earn more marks.

Attachment:- Scientific Skills Test.rar

Reference no: EM132763982

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