Reference no: EM133293001
Based on Basu, S., Greenwood, J., Jones, A. W. and Nurse, P. Core control principles of the eukaryotic cell cycle. Nature 607, 381-386 (2022).
The authors state that they "developed a time-resolved multiplexed proteomics and phosphoproteomics procedure to monitor both the amount of induced cyclin-CDK present in cells and the ability of that CDK to phosphorylate hundreds of known CDK substrates" The data resulting from this procedure are summarized in figure 2. This is a fairly dense methodological description. To unpack this, please answer the following questions?
1. What do the authors mean by time-resolved?
2. What technique/instrument is used to make these measurements and what is actually measured?
3. Why do the authors measure both protein amounts and phosphoprotein amounts?
4. How do the authors ensure they can compare data between samples with this method?
5. What is the main finding made possible by this approach?
6. What is the advantage of using this approach as compared to e.g. using an antibody against a particular phosphopeptide?