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After performing a ligation reaction between the DNA fragments and cleaved vector, you analyze the products of the reaction by gel electrophoresis.
ligation products cut with HindII Size markers
a. What DNA molecules do the two bands in lane 1 represent?
b. What DNA molecules do the two bands in lane 2 represent?
What is the dissimilarity between vag and vrg genes? How are BvgS and BvgA involved in regulation of virulence genes? What is a phase variation in B. pertussis and what causes it.
Determine what is known mechanistically about how dosage compensation occurs in mammals/humans? what gene is involved with the process?
Discuss how the unique physical and chemical properties of water contribute to the importance of water for life on Earth to survive.
While many microbes give a service to us, many are extremely dangerous. Thus far, we have reviewed many different microbes that produce a variety of diseases in body.
Provide summary the structural organization of DNA. In your answer, be certain that you identify the chemical components of the molecule, and the arrangement of the molecule
Her breath smells like acetone and her blood glucose test out at 650mg/100ml of blood. She is in acidosis. What hormone must be administered to her and why. Where may she find such DNA.
Create a stretch of DNA that will encode for five or more amino acids. Which DNA sequence will encode for at least five amino acids?
DAALAQTAHRLKGVFAMLN after phosporylation of His through RcsC the following pKas are revelead: N terminus= 9.5 and C-terminus 4.5 Asp side chain: 3.3;
Destroying the Trojan horse. Penicillin is hydrolyzed and thereby rendered inactive by penicillinase, an enzyme present in some resistant bacteria.
Determine what biological function do methylases perform in bacteria? What adaptive value do they have for a bacterium?
Provided that a substance's permeability represents the distance it can travel in a lipid bilayer per unit time, how long would it take an uncharged.
A protein that tests disulfide positive is oxidized. Spectrophotometry readings are taken for both unoxidized and oxidized protein.
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