What differs in this reaction from a traditional pcr

Assignment Help Biology

Reference no: EM13968286

You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations.

What is a multiplex PCR? What differs in this reaction from a traditional PCR?

How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.

What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?

What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?

Define incomplete 3' addition. How does this appear on an electropherogram?

What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?

Reference no: EM13968286

Questions Cloud

How long have you worked in the health care industry : How long have you worked in the health care industry? What are your current roles and responsibilities? How do you view the challenges of diversity for health care organizations?

Analyze and interpret financial statements. : Use at least three (3) quality academic resources in this assignment. Note: Wikipedia and other Websites do not quality as academic resources.

Union-by-height or union-by-size : Prove that if path halving is performed on the finds and either union-by-height or union-by-size is used, the worst-case running time is O(Mα(M, N)).

Problem regarding the disjoint sets algorithm : Show that if all of the unions precede the finds, then the disjoint sets algorithm with path compression requires linear time, even if the unions are done arbitrarily.

What differs in this reaction from a traditional pcr : What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?

Difference between an indirect and a direct cash flow state : Discuss the interrelationship of the cash flow statement to the other financial statements.

Sequence of m operations : Show how to implement all three operations so that the sequence of M operations takes O(M log N/log log N) time.

Community health needs assessment : If you are the health education director at a local hospital tasked with forming an advisory committee that will oversee a community health needs assessment, who should be invited from your community to participate and what their role(s) would be.

Determine the effects of path compression : Write a program to determine the effects of path compression and the various unioning strategies. Your program should process a long sequence of equivalence operations using all six of the possible strategies.

User Account

All Pages