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You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations.
What is a multiplex PCR? What differs in this reaction from a traditional PCR?
How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.
What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?
What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?
Define incomplete 3' addition. How does this appear on an electropherogram?
What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?
A researcher infects a bacterium with a bacteriophage and notices that the infection does not immediately bring about the destruction of the host cell. Instead, the phage genetic material is copied whenever the host reproduces. Which viral replica..
In cats, coat color is an X-linked trait where a black coat is determined by the XB allele and a yellow coat by the Xb allele. Black and yellow are co-dominant and a heterozygote has a tortoiseshell coat.
Why is it beneficial for a cell signaling pathway to be coupled to a MAP kinase cascade which essentially activates a number of MAP kinase proteins via phosphorylation?
q. hutchinson-gilford progeria is an exceedingly rare human genetic disorder in which there is very early senility and
What did you discover about malaria that you did not previously know. What are some countries doing to prevent the spread of malaria. Where is malaria most prevalent
All ribosomes are associated with the rough endoplasmic reticulum; in fact it gets its name ("rough") from how ribosomes look in the electron microscope lining its surface.
How do you determine protein expression in gene transferexperiments? How can you study receptor-mediated endocytosis in culturedcells?
In regard to cursorial locomotion and saltatorial locomotion, discuss the similarities and differences in limbs required for each.
Discuss and explain resting membrane potential and differentiate the concept of changes in polarity vs changes in concentration gradients.
Describe the various types of information that epidemiologists use to detect, interrupt and prevent epidemics. List the possible mechanisms of transmission and reservoirs of infection.
Describe the fermentation products are produced and how the seproducts are formed from pyruvate in microbial fermentation systems in the rumen or hindgut
What do the sex determination systems of Chlamydomonas and Cannabis have in common? What is different? Answer in 4-5 sentences.
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