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Design an enrichment culture medium and a protocol for the isolation and purification of a soil bacterium (e.g., Bacillus subtilis) from a sample of soil. Note possible contaminants and competitors. How will you adjust conditions of growth, and what conditions will be adjusted to enhance preferentially the growth of the Bacillus?
Offer a model to explain how the F-box protein SLF and the S-RNase mediate self incompatibility. Be sure to account for the action of these proteins in compatible and incompatible interactions.
Biochemists make stock solutions of reagents and use them to make working concentrations of buffers. Explain how to construct these stock solutions. You decide on the final volumes and show your calculations
Acquisition of a conjugative plasmid harboring antibiotic resistance genes is one of the easiest ways for bacteria to develop resistance to antibiotics.
What is an inspiratory capacity (IC) of a person with a tidal volume of 505 ml and an inspiratory reserve volume of 1950 ml? Is this person male or female.
Consider a genetic disorder in which healthy allele mutates to a recessive deleterious one with a rate of 10^-8, and the fitness cost is a reduction of the fitness by 25%.
You are analyzing the structure of a gene and find it contains a restriction site for Pst1. This site is ~1000 bp away from another restriction site for Not1.
The reactions of glycolysis are catalyzed by enzymes and obviously proceed without much problem. However, when we look at some of the individual steps, they have positive standard free energies. What are these steps and how does the process preven..
Using one single color of bead, build a homologous pair of duplicated chromosomes. Each chromosome will have 10 beads with a di%uFB00erent colored centromere in it.
express the processes of transcription and translation
Determine what functional adaptation does cartilage have over bone in making up the external framework of the nose?
Explain how turgor pressure (water concentration) regulates opening and closing of stomata. What other factors are involved in controlling stomatal opening and closing?
Discuss the greatest effect on determining which mutations will go to fixation in both a large population and a small population?
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