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What concentration of alanine is needed

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In order to undertake a spike recovery trial of a method for the analysis of alanine, you need to prepare the solution for the spike addition. Typical levels vary from 10 to 50 mg/L alanine. The analysis requires a sample of 5 mL.

a. What concentration of alanine is needed, and what volume of that solution should be added to each sample prior to analysis for the spike addition?

b. Using that spike, what is the added alanine that would be expected in the sample at 100% recovery?

Reference no: EM132409979

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