What characteristics of bacterial cells can be determined

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Reference no: EM133531202

Question 1: Why are bacterial cells generally stained for microscopic viewing?

Question 2: The exterior of most bacterial cells is rich in negative charges. Which type of stain would best adhere to such bacterial cells: a basic stain or an acidic one? Explain your answer.

Question 3: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain? Explain your answer.

Question 4: What characteristics of bacterial cells can be determined from a simple stain?

Question 5: A bacterial cell has only a small amount of peptidoglycan in its cell wall. Beyond the cell wall lies an outer membrane. If the Gram stain procedure were applied to this cell, what color would it appear to be?

Question 6: What is unusual about the cell walls of acid-fast-positive bacteria that makes them difficult to stain without heat?

Question 7: Describe the Schaeffer-Fulton endospore staining technique. Include the primary stain, mordant (if applicable), decolorizer, counterstain and visual results for endospores and vegetative cells.

Question 8: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?

Reference no: EM133531202

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