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You have isolated a new protein and are in the process of characterizing it. Several pieces of data about the protein are presented below. What can you deduce about the structure of this protein at primary, secondary, tertiary, and quaternary levels.
A) The protein elutes in a void volume on a gel filtration column with a fractionation range of 30kDa to 300kDa
B) SDS-PAGE(reduced) shows the protein has three bands with molecular weights of about 215, 18, and 22kDa.
C) Following brief treatment of the original protein with chymotrypsin, the resulting protein sample now elutes from the gel filtration column as two peaks (250, 130kDa)
D) The 250kDa sample from C) runs as a single band at 125kDa on SDS PAGE (reduced) SDS PAGE (reduced) of the other sample from C) shows it to contain 3 bands 90,22, and 18kDa.
Which term describes tonicity of the external solution. Were latitude the only control of temperature, the isotherms would run straight across the map from east to west. Describe one region of the world where this hypothetical isotherm pattern is rea..
In accordance with the Theory of Evolution, Natural Selection is mechanism through which the evolution happens. Advantageous traits survive the longest and leave the most offspring, a process termed as Natural Selection. Select an animal species.
List and explain at least three cellular features of bacteria that might be targeted to inhibit or kill a bacterial pathogen.
What is an inspiratory capacity (IC) of a person with a tidal volume of 505 ml and an inspiratory reserve volume of 1950 ml? Is this person male or female.
Set up the suitable two-by-two table and determine a measure of association between current smoking and myocardial infarction. Determine a measure of the excess risk of myocardial infarction in cigarette smokers that is attributable to their smoking.
What are the disadvantage and advantage of each and which commonly applies to hospitality industry. How is this caused by the sympathetic nervous division.
A rabbit taken from a meadow near sea level and moved to a meadow high on a mountainside would have some trouble breathing. Why give an appropriate reason.
The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar through pour plate method. The plates were incubated aerobically for 48 hours at 37C.
He says that the mere presence of DNA is not sufficient to prove the point: his cells may have adopted DNA as a useful molecule quite independently of all other known life forms. What would you do to convince him that he was wrong.
List and express at least three prokaryotic cellular features that might be targeted in this way to inhibit or kill a bacterial pathogen.
A friend of yours is wearing a bright red shirt. In terms of properties of light and pigments, describe why the shirt appears red to you.
If I wanted to test the drug to see if it made mice run faster, how could I test that and what would be my control?
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