What bacterium did you use as your test organism

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Reference no: EM132410267

Background:

A new antibiotic had just discovered recently. The effectiveness of this drug is shown below. To determine the method of action of this antibacterial chemical, you culture a bacterium in two bioreactors containing nutrient broth, add the new drug to the test broth after 20 hours, and analyze both media. Protease activity is easily measured because protease is an extracellular enzyme.

Data

Antibiotic Disk Test

Bacterium

Zone of Inhibition (mm)

Bacillus subtilis

0

Escherichia coli

10

Mycobacterium phlei

0

Pseudomonas aeruginosa

8

Salmonella typhimurium

9

Staphylococcus aureus

3

Streptococcus pyogenes

3

1) The measurement of cellular DNA is proportional to growth

2) The increase in protease suggests that protein synthesis is stopped

Questions

1) What bacterium did you use as your test organism? 

2) What is the method of action of this antibiotic?

3) Why does this antibiotic affects bacteria and not human cells?

Reference no: EM132410267

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