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To do molecular biology studies of DNA, one must develop methods to extract it. The extraction of DNA can be very complicated because of the structural and chemical complexity of cells. Eukaryotic cells are composed of many different organelles that are suspended in a complex aqueous matrix of soluble and insoluble materials. Plant cells are more complicated because they are surrounded by a rigid cell wall. Molecular biologists need to break up the cells and extract the DNA from the nucleus where most of the cell's DNA is located. We will use the strawberries to isolate DNA.
1. Why use the fruit strawberry to isolate DNA?
2. What are the materials needed to isolate DNA?
3. What are the steps in isolating the DNA from strawberries?
explain how an action potential, originating at the axon hillock, is propagated along myelinated and nonmyelinated axons.How are graded potentials made
Begin by distinguishing between osmosis and diffusion; how are they similar? In what ways are they different?how to utilize transport substances across the cell.
find out the major pathway of glycine degradation. you find that the amino acid serine contains the most radioactivity, and that both the Calpha and Cbeta carbons of the serine residue are labeled with 13C. Explain these observations.
What is left-sided and right-sided heart failure? Which of these symptoms reflect left-sided heart failure and which reflect right-sided heart failure? Why with reasons.
express "transamination" and "oxidative deamination"
The "Primordial soup" includes the mixture of elements and gases which are thought to have started life on the Earth. Speculate on how, over the course of evolution, these complex molecules would eventually form the complex life forms such as human..
Calculations must be made assuming that the minimum significant number has four significant digits. If all of phosphorus in a 3.00 gram sample of MgNH4PO4 is extracted and used to make a diphosphorus pentaoxide, what mass of P2O5 could be produced.
What is basics of epidemiology, brief description on Understanding basics of epidemiology
compute the change in G value for the reactions
Suppose you have a light chain genome with 64V regions and 8J regions. If you had repeated attempts at recombination, how many dissimilar attempts would be made when the middle region of both V and J is the segment chosen at each recombination.
The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Utilize the results from replica-plating to reply the questions.
You are having a 2.5 mg/ml solution of an amino acid ( mol.wt. = 100). You are need to add the 0.5 µmoles into a test tube. Specify the amount you would pipette?
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