What are the cq values of each of our patient samples

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Reference no: EM133450729

Questions:

1. Describe how to perform a melt curve? In your results, what did the melt curve tell you about the products of the PCR reaction?

2. What are the CQ values of each of our patient samples?

3. Which patients had the highest viral load?

4. Name three factors to consider when designing pairs of primers to be used for a PCR reaction.

5. Diagnosis of HIV has traditionally relied upon a western blot rather than PCR. If PCR is so sensitive, why do you think this is the case? Contrast what information a western blot provides and what a PCR reaction provides. What are the benefits and disadvantages of each test?

Reference no: EM133450729

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What are the cq values of each of our patient samples : Describe how to perform a melt curve? In your results, what did the melt curve tell you about the products of the PCR reaction?

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