What are heterophile antibodies

Reference no: EM133677708

Assignment:

Two potential interferences with "sandwich" ELISA include heterophile antibodies and the hook effect.

a) What are heterophile antibodies and how does the presence of heterophile antibodies interfere with the results of a "sandwich" ELISA (2 marks).

b) What is the hook effect as it relates to the "sandwich" ELISA technique and how does this effect interfere with the performance of a "sandwich" ELISA (2 marks).

c) What necessary step may need to be performed prior to sample analysis to control for the high dose hook effect (1 mark)?

Reference no: EM133677708

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