What accounts for the different final locations

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Reference no: EM133313970

Question 1: What is the composition of a DNA fragment? That is, what is a DNA fragment made of?

Question 2: Using the lane numbers on the electrophoresis instrument, list the samples from smallest DNA fragment to largest DNA fragment. Label the largest and smallest

Question 3: What accounts for the different final locations of the different size DNA fragments? That is, why do different sized DNA fragments end at different locations on the electrophoresis instrument?

Question 4: Does the crime scene sample contain bands in common with the student? Explain why or why not.

Question 5: Which suspect (if any) CANNOT be excluded from suspicion? Explain your answer.

In the table below indicate the number of DNA fragments each sample has in common with the child/calf sample (Lane 2). Note: Sample 1 is in Lane 1 of the gel, etc.

Question 6: Which sample is from the father of the calf? Explain your answer.

Question 7: How many bands does the calf have in common with its mother? How many bands does the calf have in common with its father? Propose a plausible explanation for the number of bands from each parent.

Question 8: After adding the probe and filter, what additional information have you learned about the genotype of the calf?

Question 9: Given that the probe pairs with a specific DNA strand in the matrix, what can you conclude about the nucleotide sequence of the Baby's DNA fragment that pairs with the probe sequence?

Question 10: Discuss reasons that it might be useful to find out whether a newborn carries a specific gene or allele.

Question 11: explain why it is necessary to include only one chain-terminating/synthesis-terminating nucleotide in each well of the electrophoresis instrument.

Question 12: Determine the DNA sequence by analyzing the location of each nucleotide across the four lanes of the electrophoresis instrument. Provide the DNA sequence as your answer here.

Question 13: What is the significance of finding that DNA sequences are highly similar for two different species?

Question 14: Below, align the newly determined DNA sequence with the sequences of the three known plant species (see data under the Activity Form tab). Next, compute the percent alignment of the bases for the three comparisons.

Question 15: A biologist at World University used Procedure IV to conduct an additional study and found the sequence following below. Using your knowledge of central dogma generate the amino acid sequence that results from this sequence.

Reference no: EM133313970

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