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Your intern spills the entire bottle of 0.1 M, pH 5.00 buffer that came with an enzymatic assay kit for malic acid. Rather than buying a new kit, you ask your intern to make an acetic acid/acetate buffer with this pH. Your intern starts by adding 2.86 mL acetic acid and 4.10 g sodium acetate to a 1.00 L volumetric flask and diluting to the mark with water.
Acetic acid: pKa 4.76, density 1.05 g/mL
a. Conjugate acid and conjugate base combined, does the overall buffer concentration (M) match the buffer provided with the kit?
b. What volume (mL) of 1 N NaOH is required to adjust the pH to the desired 5.0?
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In the first step of the synthesis of PABA, why was it necessary to add sodium acetate to have reaction of p-toluidine with acetic anhydride?
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