Visualizing dna for emsa, You are investigating YFP, a

Visualizing dna for emsa

Reference no: EM1398037

You are investigating YFP, a transcription factor that binds directly to DNA. You know that it recognizes the following sequences. (Only one strand of the DNA is shown, but double-stranded DNA is required for your factor to bind).
5' ATTGCATAGTATCCTATT 3'
You have purified your factor and you would like to do a gel shift assay (EMSA).
A. Describe how you would visualize your DNA for your EMSA.
You combine the protein and DNA and run it on the gel. You see the expected shift in the DNA upon addition of your factor. We will consider this as the baseline conditions.
Explain the following in both words and with figures showing your expected results:
B. What will happen if you increase the amount of your protein in the gel shift?
C. You perform the assay again and add 100 fold excess unlabeled DNA corresponding to your fragment to the baseline condition. What will happen in this case?
D. You perform the experiment again but add 100 fold excess of an unlabeled 18 base pair fragment that is not recognized by your factor to the baseline condition. What affect will this have in the experiment?
E. What experiment would you choose to do next with YFP?

Reference no: EM1398037

Questions Cloud

What is the rationale for vaccination and quarantine : Lagniapposis is caused by a microparasite, Boudreauxia thibodauxi, and is transmitted directly by coughing and sneezing. It is highly transmissible and very virulent.

Write essay to explain cesae chavez was effective leader : Write down essay about how cesae chavez was effective leader, create the thesis for dbq in the initial paragraph. Explain his self-sacrifice, persistence and courage.

Performing a two-sample z-test for proportions : Perform a two-sample z-test for proportions to test if men are more likely than women to make online purchases of books.

Determining interpretation of anova results : An occupational health study was carried out to evaluate the health status of workers. The response is forced expiratory volume (FEV), a measure of respiratory function

Visualizing dna for emsa : You are investigating YFP, a transcription factor that binds directly to DNA. You know that it recognizes the sequences.

Calculate the shannon-wiener index : calculate the Shannon-Wiener index (H0) for each plot. I've broken the data above into three separate tables for your calculations. Fill in the following three tables and calculate H0 for each plot.

Carrying a girl on a large plank : Two men are carrying a girl on a large plank that is 10m long and weights 200N. If the girl on a plank weighs 445N and sits 3m from one end, how much weight must each man support?

Explain basic components of religious traditions : Explain these basic components of religious traditions and their relationship to sacred what religious traditions say it teaching text doctrine stories.

What is the percent composition of this solution : How much calcium chloride is needed to make 350 mL of a 50 mM CaCl2 solution? What is the percent composition of this solution.

User Account

All Pages