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Use of molecular methods in clinical microbiology

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Reference no: EM132644612

Name one advantage and one disadvantage of the use of molecular methods in clinical microbiology.

What is the signal detected in qPCR procedures? and What two methods can be used to analyze the quality and specificity of amplification in a qPCR?

Which of the following controls is critical for ensuring that amplification is occurring in a patient sample and that the lack of PCR product is not due to the presence of polymerase inhibitors? (2pts)

a. Reagent blank

b. Sensitivity control

c. Negative control

d. Amplification control

An EWS-FLI-1 mutation was detected in a solid tumor by qRT-PCR. Which of the following does this result support? (2pts) a. Normal tissue

b. Ewing sarcoma

c. Inherited breast cancer

d. Microsatellite instability

Home-brew" laboratory-developed tests: (2pts)

a. require premarket approval.

b. require no review prior to implementation.

c. present no risk of human injury.

d. require approved validation.

Reference no: EM132644612

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