Understand the fundamental basis of chromatography

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CH3202 Applications of Molecular Spectroscopy

Knowledge and Understanding

Question 1. Describe the underlying physical principles behind modern spectroscopic techniques;

Question 2. Describe qualitatively and quantitatively the information provided by 1D and 2D NMR, IR, and UV-vis spectroscopies and mass spectrometry;

Question 3. Relate the appearance of IR, UV-vis, 1D and 2D NMR and mass spectra to the relevant structures and physical properties of the molecular species;

Question 4. From an appreciation of molecular form and structure predict the appearance of IR, UV-vis and NMR spectra for a wide variety of organic and inorganic molecules;

Question 5. Understand the fundamental basis of chromatography and the physical origins of separation;

Question 6. Discuss column design, support phase performance and ‘theoretical plates';

Question 7. Describe the common methods of post-chromatographic product detection based on UV-vis and MS analyses;

Question 8. Prepare samples, operate spectrometers, and obtain qualitative and quantitative information from IR and UV-vis spectra.

Intellectual Skills

Question 1. Deduce appropriate chromatographic purification procedures and spectroscopic methods for identifying molecular compounds;

Question 2. Analyse and interpret spectroscopic data to deduce detailed information about the molecular structure and physical properties of inorganic and organic compounds;

Question 3. Utilise appropriate combinations of spectroscopic data to identify molecular structures.

Reference no: EM133112169

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