Two-hybrid screens to test your hypothesis

Assignment Help Biology
Reference no: EM133307432

You are investigating the role of Gene M in motor neurons. Through coimmunoprecipitation, ChlP seq, and mass spectrometry, you have identified three proteins (A, B, and C) that seem to associate with your promoter and each other in a cell culture model of motor neurons. You suspect, however, that one of these interactions is an experimental artifact, and only two of the proteins associate under cytosolic conditions.

Design a series of two-hybrid screens to test your hypothesis (drawings may make this easier) Think about the selection conditions you need in order to know you have the two plasmids of interest, as well as something else to act as a reporter for interactions between two interacting proteins.

Reference no: EM133307432

Questions Cloud

Define and identify the high status and power of the queen : ART 1100 University of West Georgia define and identify the high status and power of the queen through: the furniture visible around her. her clothes her beauty
Proteins coded by these genes than do males : Typically, however, females do not produce more of the proteins coded by these genes than do males.
What differences in the use of visuals text and audio : What differences in the use of visuals, text, and audio might you consider for a lesson on how to use exercise equipment versus how a virus causes the flu?
What are things we have usually observed in well programs : What are the things we have usually observed in well-managed programs?What are important skills that every good program manager should possess?
Two-hybrid screens to test your hypothesis : Design a series of two-hybrid screens to test your hypothesis.
Discuss remuneration and rewards social security : Discuss how remuneration and rewards Social Security Organisation (SOCSO), also known as PERKESO (Pertubuhan Keselamatan Sosial), was formed under the Employees
Discuss how that characteristic of that faith tradition : RST 1460 Madonna University discuss how that characteristic of that faith tradition could be integrated into the American experience - either as something
Which marfan is inherited between generations : What are the ultimate results and consequences with this disorder? Develop a hypothetical example in which Marfan is inherited between generations.
How would you describe the task of the church in the world : In general terms, how would you describe the task of the Church in the world and In specific terms, name three or four of the challenges faced by the early

Reviews

Write a Review

Biology Questions & Answers

  Describe how vesicular transport occurs in cells

What would happen if one were to inject both the R strain and heat-killed S strains into a host organism such as the mouse.

  What was the genotype of the original brown parent

Brown color in mice is dominant over albinism. In a given cross between a brown and albino, six brown and five albino offspring are produced. What was the genotype of the original brown parent?

  Prognostic impact of gene fusion in blood cancer

Discuss the pathological and prognostic impact of gene fusion in blood cancer.

  Identify the gene and make large amounts of the protein

How could you use a cDNA library to identify the gene and make large amounts of the protein in bacteria? Please include all the steps you would take and what tools

  The formation of big molecules from small repeating units

1.amino acids are the building blocks fora.proteins.b.steroids.c.lipids.d.nucleic acids.e.carbohydrates.2.the formation

  Explain the structures and functions of the male and female

create a 7- to 10- slide microsoftreg powerpointreg presentation with detailed speaker notes about a reproductive

  Describe the anatomy and basic physiology of the system

Describe the anatomy and basic physiology of the system. Articulate what makes the system unique/primary focus. Choose a common disorder of the system.

  Why do you think taxonomy is important

Differentiate between archaea, fungi and bacteria. Why do you think taxonomy is important?

  How does acid rebound occur

How does acid rebound occur? What are the therapeutic actions of sulcrafate and misoprostol?

  What are its symptoms and cause

Choose a lung disease, Bronchitis, Asthma, COPD, Emphysema and name examples, causes, and treatments

  Outline the flow of blood through the heart

Outline the flow of blood through the heart. Explain the function of valves as you proceed. What are the possible causes of blocked coronary arteries. What could result if the condition is not treated. How can a coronary blockage be treated.

  Boss was misinterpreting statistical data

Describe how you would handle a situation at a human services agency where your coworker or boss was misinterpreting statistical data.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd