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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
What is the name of the enzyme that performs the lysis step in glycolysis, spliting a 6 carbon sugar molecule into 2-3 carbon molecules?
Discuss the difference between DNA methylation and histone methylation? I understand that both include the repression of genes and that DNA methylation occurs at CpG islands.
What does it mean to be an "multicellular organism"? The cellular slime mold Dictyostelium discoideum exists initially as a collection of separate cells moving over the forest floor.
q1. ebv infects b cells and some epithelial cells. ebv enters via the cd21 part of b cell coreceptor.the atypical
Evaluate the objectivity and legitimacy of biology information found in articles and online. Compare article with multiple sources of information about the same concepts
Individuals with diabetes mellitus produce urine that is concentrated and higher in volume than the urine of healthy individuals. Explain why these two characteristics are observed in diabetics.
Researchers are developing blood substitutes to meet the demand for emergency transfusions. What would be the effect of artificial blood in which binding of O2 is not altered by acidity?
Which layer of the atmosphere contains the ozonelayer?
Write an Essay paper on the Skin Disorder. Describe the structure and function of the skin (integumentary system) and related diseases.
Discuss and explain the dependent and independent variables in the water quality vs. fish population experiment?
Importance Of Bicarbonate In Human Body
What is the difference between respiration meaning gas exchange and cellular respiration - what is the chemical equation of the aerobic cellular respiration?
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