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Assume you have discovered a new Xenopus gene that codes for a RNA binding protein. You are not sure what its function is but you are interested in finding out as much as you can about the protein's role in development of the embryo (from oocyte to tailbud stage) so that you can publish your results. You have tentatively called the protein Xp65 (Xenopus protein 65 kDa).
A) How would you go about determining where and when the gene is expressed in the embryo? Make sure that you think about all the important information that you would want to know to understand when and where this gene product might be functioning. B) Once you have established the expression profile, what might you want to do to help you understand how the gene effects development? Explain what experiments you might run, and why.
C) The protein turns out to have a role in transcriptional activation during gastrulation in addition to its RNA binding activities. What might you do to determine the targets of this protein (what it turns on)?
D) You find that the gene has an ortholog in Drosophila. What might you want to do in Drosophila that you cannot do in Xenopus? Explain why this might work in Drosophila but not Xenopus.
Clarify how the procedure of meiosis assures that the chromosome number of a species remains the same from generation to generation.
A hurricane in Caribbean devastates a small isolated island, destroying all but about a dozen of once dominant turpentine trees on the island.
Using examples, discuss types of genetic mutations that result in faulty proteins and disease,
A membrane which is permeable to an uncharged molecule X separates two solutions. Solution A contains a 100 mM solution of X and B 10 mM. If the permeability constant for X is 5 cm/s.
the required reagents involved in making the PCR work.
During gym class Sally noticed that her friend Melissa always ran faster than her. Sally knew that they exercised equally, so she wondered what could cause Melissa to run so fast.
If a double heterozygous plant were crossed with a plant that has green stems and red fruit, what offspring phenotypes would be expected and in what proportions.
are ancestry dna tests(mtDNA,Y-chromosome STR test) reliable.
What other diseases are thought to be caused by the point mutations? What do point mutations do in those diseases.
why materials move from high to low concentration. Isn't this just a waste of energy.
Which extinct species should be the best candidate to serve as the out-group for clade whose common ancestor occurs at position 2.
A Drosophila experiment analyzing linkage of X-linked genes studied a recessive eye mutation, a recessive wing-vein mutation, and a recessive bristle mutation
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