Total dilution of the original bacterial culture

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Suppose someone starts a broth culture of E. coli at an initial concentration of 5,000 cfu/mL. Suppose they mix 2 mL of culture with 8 mL of sterile saline. What dilution of the bacterial culture have they prepared? Now mix 2 mL of the dilution with 8 additional milliliters of sterile saline. What dilution of the dilution have they created? What is the total dilution of the original bacterial culture in the final tube?

Reference no: EM132797314

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