Topic - Effect of eco-fungicide on soil bacterium

Reference no: EM132314759

Microbiology Assignment -

Topic - Effect of eco-fungicide on soil bacterium, P. putida

Experiment report - Use the references calculate the CFU include the calculation.

Background: Hypothesis: The eco fungicide, with the potassium bicarbonate 3G/L as an active ingredient, has a negative impact on decreasing the life of the beneficial soil Pseudomonas putida bacteria

Treatment of soil bacteria, Pseudomonas putida, with 100 µL of "Eco-fungicide" (3g/L) for 1 hour will lead to fewer CFU than 1 hour treatment with 100 µL of "Eco-fungicide" (0.3g/L). Additionally, 1 hour treatment with 100 µL of "Eco-fungicide" (0.3g/L) will lead to fewer CFU than 1 hour treatment with 100 µL of water.

Null Hypothesis: Treatment of soil bacteria, Pseudomonas putida, with 100 µL of "Eco-fungicide" (3g/L) for 1 hour will lead to the same CFU as 1 hour treatment with 100 µL of "Eco-fungicide" (0.3g/L). Additionally, 1 hour treatment with 100 µL of "Eco-fungicide" (0.3g/L) will lead to the same CFU as 1 hour treatment with 100 µL of water.

Variables:

Dependent Variable - Growth of soil bacteria Pseudomonas putida

Independent Variable - "Eco-fungicide" (active ingredient Potassium Bicarbonate)

Controlled Variable - Quantity of solvent

Control(s) -

Experimental Design:

-- flowchart

Note that you should be able to explain how you have determined which dilutions to prepare and which samples you plan to plate when you submit your proposal for marking.

Attachment:- Assignment Files.rar

Reference no: EM132314759

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