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Your friend, who is an engineering major, is interested in the mechanical properties of extracellular matrix and wants to study how secreted collagen molecules assemble into a collagen fiber. As part of his studies, he has been trying to speed up the rate at which cells can produce the collagen protein and is considering reducing the size of the propeptide that is attached to the collagen chain such that it contains only the ER targeting sequence as well as the site for cleavage of the propeptide. To his surprise, this modification causes collagen to be degraded inside the cell. Can you explain why?
What is a "Holliday Structure"? Describe the differentially resolved heteroduplexes and recombinants.
Glycogen is a polymer, so it has more energy than an equivalent number of glucose molecules. How does the cell find the energy necessary to make this storage product?
In cases of severe iron deficiency, cellular respiration can not proceed normally. Disregarding problems related to hemoglobin or red blood cells, at what stage of cellular respiration would lack of iron cause the greatest problems?
For an enzyme (5 µM) , the following initial velocities have been reported depending on the substrate concentration.
Suppose you start a firm where you genotype individuals determine which alleles they carry for a set of genes and help them with their training for marathon.
A 3-year-old child is admitted with primary nephrotic syndrome. What signs and symptoms would be identified? What are the mechanisms behind these manifestations? What is the primary treatment for this syndrome and why?
You have a 1mM stock solution of Arsenic to treat culture cells in a liquid medium. You need to treat your cells with Arsenic such that it has a final concentration of 5nM once it has been added to the cells.
MacConkey Agar gives two results: growth and color change. What does each of the two results tell you about the characteristics and/or biochemical abilities of the organism being tested?
How is the villus the functional unit of the small intestine? In your answer, include a discussion of its structure and function and the enzymes found there. What is the importance of surface area to the functioning of the villi?
Two protein kinases, PK1 and PK2, work sequentially in intracellular signaling pathway. You construct cells that contain inactivating mutations in the genes that encode either PK1 or PK2
As fate would have it, Alonso is taking a medication that specifically blocks muscarinic acetylcholine receptors. Bernd is taking a medication that specifically blocks nicotinic acetylcholine receptors.
How can DNA be extracted and analyzed from burnt bones or well preserved bones using PCR, STR, and amplification of mitochondrial mtDNA?
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