This is actually the sum of two half reactions the sum of

Reference no: EM13633974

he synthesis of a phosphodiester bond involves condensation of a5'- phosphoryl group on one nucleotide with a 3' hydroxyl group onan adjacent nucleotide. DNA Ligases catalyze this reaction bycoupling condensation to cleavage of a phosphoanhydride bond fromeither NAD+ or ATP.

The overall reaction catalyzed by T4 DNA ligase is seen in thisequation:
ATP + nicked bond -> AMP + PPi +phosphodiester bond

This is actually the sum of two half reactions. The sum of ahydrolysis reaction coupled to a condensation reaction isthermodynamically equivalent to the ligase reaction represented bythe overall reaction (above).

Why does the ratio of nicked to diester bonds increase as more AMPis added to the system?

Reference no: EM13633974

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