The total strand concentration c that is duplex is defined

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Reference no: EM13345655

Suppose a DNA duplex is not self-complementary in the sense that the two polynucleotide strands composing the double helix are not identical. Call these strands A and B. Call the duplex AB. Consider the association equilibrium of A and B to form duplex AB A + B-> AB Assume the total strand concentration is C and, initially, A and B have equal concentrations; that is, CA,0 = CB,0 = C/2. Obtain an expression for the equilibrium constant at a point where the fraction of the total strand concentration C that is duplex is defined as f. If the strand concentration is 1.00 X 10^-4 M, calculate the equilibrium constant at the melting temperature.

Reference no: EM13345655

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