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The Entner-Doudoroff pathway, which is used by some microorganisms, including the talented Sinorhizobium meliloti:1) Glucose + ATP ? Glucose-6-phosphate + ADP2) Glucose-6-phospate + NADP+ ?6-Phosphogluconolactone + NADPH + H+3) 6-Phosphogluconolactone + H2O ?6-Phosphogluconate4) 6-Phosphogluconate ?2-Keto-3-deoxy-6-phosphogluconate5) 2-Keto-3-deoxy-6-phosphogluconate ?Glyceraldehyde-3-phosphate + Pyruvate6) Glyceraldehyde-3-phosphate + NAD+ + Pi ?1,3-Bisphosphateglycerate + NADH + H+7) 1,3-Bisphosphateglycerate + ADP ?3-Phosphoglycerate + ATP8) 3-Phosphoglycerate ?2-Phosphoglycerate9) 2-Phosphoglycerate ?Phosphoenolpyruvate + H2O10) Phosphoenolpyruvate + ADP ?Pyruvate + ATPA. Which of the reactions above are also found in the glycolysis pathway we are studying in class?B. How many (net) moles of ATP are produced per mole of glucose in the Entner-Doudoroff pathway? (Don't include any ATP made during the citric acid cycle or by oxidative phosphorylation.)C. Assuming the organism uses pyruvate dehydrogenase and the citric acid cycle for aerobic growth, how many (net) moles of ATP (or ATP + GTP) would be produced per mole of glucose by substrate-level phosphorylation as a result of the action of the Entner-Doudoroff pathway and the citric acid cycle?D. Identify the step(s) in the pathway above in which the metabolite is oxidized. Explain how you recognized that an oxidation-reduction reaction was taking place in this step (these steps).E. Assuming the organism has pyruvate dehydrogenase, how many moles of acetyl-CoA would be produced per mole of glucose when it is growing aerobically using the Entner-Doudoroff pathway? Explain how you got your answer.F. Again assuming the organism uses pyruvate dehydrogenase and the citric acid cycle for aerobic growth along with the Entner-Doudoroff pathway, how many moles of NADH plus NADPH would be produced per mole of glucose metabolized? Explain how you got your answer.
Compare and contrast the structure and function of the circulatory systems in insects and in mammals. Explain why each animal's system works for that animal. Don't ignore gas exchange; it's connected with circulation.
Explain the possible fates of DNA that in brought into the recipient cell during generalized transduction.
Assume if there is no color change after the addition of the reagents noted in #4, zinc is added to the culture. What purpose does thezinc powder serve and What is the oxygen requirement of organisms that are able to reduce nitrates?
Describe the event that are invovled in the release of neurotransmitter from nreve endings?
Assume you identify a recessive mutation in the AP endonuclease in yeast. During the diploid phase of their lifecycle, the yeast are viable but haploid strains die rapidly.
What is the probability that the fetus has inherited the disease allele D if the distance between the D locus and the microsatellite marker locus is: (A) 0 map units? (B) 2 map units? (C) 50 map units? Please explain how to do it. I already have t..
Determine which population size exhibited the greatest amount of variation in allele frequency during these stimulations?
A very important part of the paper is your own opinions and ideas, based on the research you have read. Indicate which sources you agree with and which you do not, and explain your answer.
In 2010, under its WaterSense program, EPA began promoting showerheads that produce still-lower flows of two gallon every minute.
DNA sequences differ between species due to substitution events, i.e. when new mutations arise and become fixed by drift or selection in one of the species.
Oxygen is the final electron acceptor of this pathway. Glycolysis is the only energy yielding step of this pathway.
A body was discovered and diagnosed as having died due to oxygen deprivation. Explain why the toxicology tests included a test for cyanide poisoning.
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