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The bacterium Helicobactor pylori naturally grows in human and animal stomachs at pH about 2. It will grow also at pH 2 and pH 7 in culture media. It's genome sequence has been determined and annotated. You are assigned to determine which genes, if any, are highly expressed and which are repressed by growth at low pH. How would you do that? [you should be able to do so in three or four major steps but you may lsit more if you wish.]
What organelles would you expect to find in exaggerated quantities in pancreaticacinar cells? What role does each of the organelles serve?
Which has the lower concentration of hydrogen ions, vinegar at pH 3 or tomato juice at pH 4? How many times fewer hydrogen ions? Which is more acidic?
A cell whose cytoplasm has a concentration of 0.02 molar glucose is placed in a test tube of water containing 0.02 molar glucose. Assume that glucose is not actively transported into the cell, which terms describes the tonicity of an external solutio..
Before this module evaluation you were very nervous. Consider a snack might help, you ate a big bowl of pasta and some bread a couple of hourse before you started this quiz.
Can enhancers work in trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA. Propose an experiment to test this. Be sure to discuss any appropriate control experiments.
Research how a SPECIFIC biotechnology application within the above GENERAL examples (or ones not listed above) is used today. Example: Insulin production in bacteria is an example under the general topic of recombinant organisms.
which baltimore group are the retroviruses in? which baltimore group are the positive-stranded mrna bacteriophages in?
Enzymes are typically required to catalyze reactions that would be very unlikely to occur on their own. Suppose that the activation energy, Ea, for the uncatalyzed cheese reaction at 20C is 18kcal/mol.
Using the diagnostic and statistical manual of mental disorders, choose a behavioral disorder, identify a candidate gene, and explain how a mutation in it might cause the phenotype.
Explain why proteins are superior to both RNA and DNA for structural and enzymatic function?
What is the control in a experiment where a bank teller has brown eyes and brown hair and is taller than all the other bank tellers.
How would the Km change for methanol compared to ethanol and how would the Km's of methanol and isopropanol compare.
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