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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to the mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in tetracycline resistance gene. This mixture is incubated for numerous hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using the technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria containing a plasmid into which the eukaryotic gene has integrated will grow in what?
Mimulus lewisii and Mimulus cardinalis are two plants that occupy the same habitat but do not interbreed in nature. Though, they can be readily crossed in the laboratory to produce fertile offspring. Which is a likely reason that prevents interbreedi..
Explain why bacteria do not have apoptotic signaling pathways.
Black coat of animal is dominant; white is the alternative recessive. While true-breeding black is crossed to true-breeding white, what is the possibility that a black F2 is heterozygous.
In maize, a dominant allele (A) is essential for seed color as opposed to colorless (a). Another gene has a recessive allele (w) which results in waxy starch, as opposed to normal starch (W).
Frank found himself in the midst of an ethical dilemma at work, and was using an ethical decision-making matrix, to address his work and personal values. Frank was most likely in which phase of the Ethical Decision-Making Framework.
Cyanobacteria grow prolifically in streams and lakes with low oxygen levels and high nutrient concentrations. How may the presence or absence of cyanobacteria be used as an index of pollution in lakes.
Show the lines of current flow to unexcited parts of the cable. Elucidate how these local currents cause the action potential to propagate. A common mistake is to confuse the currents that flow throughout the action potential the voltage-clamp curren..
How to comprise the genotypes of both parents. The pronghorn of the United States and the saiga antelope of the central Asian steppes live in similar habitats and have alike adaptations. Which of the features would you be surprised to discover in a s..
Which receptors were monitoring the drive, still the conscious part of your brain was asleep? Explain the proper answer.
What advantages do the O-Scale System and the Canadian Physical Activity, Fitness and Lifestyle Approach-Health Body Composition Assessment procedure has in evaluation to percent body fat predictions from skin folded measurements, when used in indivi..
Assume n-butylmalonate is added to an aerobic suspension of kidney cells using glucose exclusively as fuel. Predict, with description, the effect of this inhibitor on.
The use of PCR and genetic approaches in biotechnology
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