The affinity for ag for sulfhydryl groups is so great that

Assignment Help Chemistry

Reference no: EM13637019

Many enzymes are inhibited irreversibly by heavy-metal ions such asHg2+, Cu2+, or Ag+, which can react with essential sulfhydrylgroups to form mercaptides, e.g.,
E-SH + Ag+ → E-S-Ag + H+
The affinity for Ag+ for sulfhydryl groups is so great that Ag+ canbe used to titrate -SH groups quantitatively. Just enough AgNO3 wasadded to a 10 mL solution containing 1.0 mg/mL of pure enzyme tocompletely inactivate the enzyme. A total of 0.342 ?mol AgNO3 wasrequired.

(a) Calculate the minimum molecular weight of the enzyme.

(b) Why does the value obtained in this way give only the minimummolecular weight?

Reference no: EM13637019

Questions Cloud

What must be his average speed if he is to meet the ball : a soccer ball is kicked from the ground with an initial speed of 19.1 ms at an upward angle of 45deg. a player 50.0 m

Examine the ir spectrum of aspirin several significant : examine the ir spectrum of aspirin. several significant signals have been identified on the spectrum. assign these

Finally c determine the minimum sample period in s to avoid : for the deteministic signal yt-23sin6pit4sin18pit sketch the amplitude-frequency spectrum of yt a when the sinal is

Assignment the final project for this module is a : the final project for this module is a consultancy report to anthonys orchard an expanding apple orchard and

The affinity for ag for sulfhydryl groups is so great that : many enzymes are inhibited irreversibly by heavy-metal ions such ashg2 cu2 or ag which can react with essential

Car 1 is speeding up with an acceleration of magnitude : two cars drive on a straight highway. at time car 1 passes mile marker 0 traveling due east with a speed of 20.0ms .

Determine athe fundamental frequency in hz contained in the : consider the signal yt24sin3pit3cos3pit with t in units of seconds. determine athe fundamental frequency in hz

A solution of h2so4 with unknownconcentration was titrated : a solution of h2so4 with unknownconcentration was titrated with the standard base with .0233molarity. a volume of

What is the recoild speed of the person and the skateboard : a 58-kg person holding two 0.9-kg bricks stands on a 2.6-kg skateboard. initially the skateboard and the person are at

User Account

All Pages