The affinity for ag for sulfhydryl groups is so great that

Reference no: EM13637019

Many enzymes are inhibited irreversibly by heavy-metal ions such asHg2+, Cu2+, or Ag+, which can react with essential sulfhydrylgroups to form mercaptides, e.g.,
E-SH + Ag+ → E-S-Ag + H+
The affinity for Ag+ for sulfhydryl groups is so great that Ag+ canbe used to titrate -SH groups quantitatively. Just enough AgNO3 wasadded to a 10 mL solution containing 1.0 mg/mL of pure enzyme tocompletely inactivate the enzyme. A total of 0.342 ?mol AgNO3 wasrequired.

(a) Calculate the minimum molecular weight of the enzyme.

(b) Why does the value obtained in this way give only the minimummolecular weight?

Reference no: EM13637019

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