Reference no: EM132751411
PREPARATION OF CULTURE MEDIA Methodology:
Surface-Disinfection of container
Disinfect glass/plastic container and lid by soaking them with bleach solution (1 cap bleach: 100mL water) for 10 minutes. Dry the container by placing them over a clean tissue. Cover the container with lid without touching the inner part of the container and bottom part of the lid. Ensure that hands are thoroughly washed with soap and water, or if available, gloves are used while performing this part of the experiment. The container must remain tightly closed until needed.
Preparation of Agar media
Cut a potato in half and boil it in 100mL distilled water for 15 minutes (use plastic bottles with indicated volume to measure water).
Remove the potato and filter the water using clean cloth or gauze. Add a teaspoon of agar powder and completely dissolve it by boiling. Add small amount of distilled water if the volume decreases due to evaporation. Cover the pot while boiling.
Pour-plating
Ask for assistance for this part of the experiment. Quickly pass the circumference of the container/lid area over a flamed candle. Open the lid of the container partially and pour the media inside. Fill about half or ¾ of the container. Tightly close the container and wait for the media to solidify. Wiggle gently, but do not open the container, occasionally to check if the media has successfully solidified. Invert the container.
*If refrigerator is available, the media can be stored but must be placed in inverted position. Or the learner can proceed to the next experiment.
Research the significance of the following:
a. Soaking the container in bleach
b. Keeping the container closed except when pouring the medium; partially opening the container during pour-plating
c. Use of potato in the experiment
d. Inverting
the container after the media had solidified