Suggest adding all four nucleotides from the pcr kit

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Your labmate's ligation reaction didn't work. The regular ATP had run out, so he fueled the reaction with dATP from the PCR kit. What advice can you give him?

A. Suggest trying one of the other nucleotides from the PCR kit to drive the ligation reaction.

B. Suggest adding all four nucleotides from the PCR kit.

C. Add heat to the ligation reactions to provide energy, instead of ATP.

D. Remind Sam that dATP is not the same as ATP.

Reference no: EM132751211

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